HVEM Protein (AA 39-202) (Fc Tag)
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- Target See all HVEM (TNFRSF14) Proteins
- HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 39-202
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HVEM protein is labelled with Fc Tag.
- Purpose
- Human HVEM/TNFRSF14 Protein
- Sequence
- Leu39-Val202
- Characteristics
- Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu39-Val202.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human LIGHT Trimer, His tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 1.2μM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product TNFRSF14 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
- Alternative Name
- HVEM (TNFRSF14 Products)
- Synonyms
- ATAR Protein, CD270 Protein, HVEA Protein, HVEM Protein, LIGHTR Protein, TR2 Protein, Atar Protein, HveA Protein, Hvem Protein, Tnfrs14 Protein, TNF receptor superfamily member 14 Protein, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) Protein, Tnfrsf14 Protein, TNFRSF14 Protein
- Target Type
- Viral Protein
- Background
- Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.
- Molecular Weight
- 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
- Pathways
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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