ERBB4 Protein (AA 26-651) (His-Avi Tag)

Catalog No. ABIN7274805

Product Details

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Protein Type: Recombinant

Protein Characteristics: AA 26-651

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ERBB4 protein is labelled with His-Avi Tag.

Purpose: Human Her4/ErbB4 Protein

Sequence: Gln26-Pro651

Characteristics: Recombinant Human Her4/ErbB4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln26-Pro651.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Her4 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 34.7ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Alternative Name: Her4 (ERBB4 Products)

Synonyms: erbb4 Protein, si:ch211-227n20.1 Protein, Her4 Protein, c-erbB-4 Protein, HER4 Protein, p180erbB4 Protein, erb-b2 receptor tyrosine kinase 4 Protein, erb-b2 receptor tyrosine kinase 4a Protein, v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) Protein, erb-b2 receptor tyrosine kinase 4 L homeolog Protein, receptor tyrosine-protein kinase ErbB-4 Protein, ERBB4 Protein, erbb4a Protein, erbb4 Protein, erbb4.L Protein, Tsp_13582 Protein, Erbb4 Protein

Background: Her4, a member of the EGF receptor family, plays a variety of roles in physiological and pathological states. Genetic studies have indicated a link between Her4 and type 2 diabetes and obesity. Her4 may play an important role in glucose homeostasis and lipogenesis. Her4 deficiency-related obesity and adipose tissue inflammation may contribute to the development of MetS.

Molecular Weight: 72.7 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway