TNFRSF18 Protein (AA 20-156) (His tag)
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- Target See all TNFRSF18 Proteins
- TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-156
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Origin
- Cynomolgus
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TNFRSF18 protein is labelled with His tag.
- Purpose
- Cynomolgus GITR/TNFRSF18 Protein
- Sequence
- Gln20-Pro156
- Characteristics
- Recombinant Cynomolgus GITR/TNFRSF18 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln20-Pro156.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus GITR, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. The affinity constant of 0.21 μM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product TNFRSF18 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))
- Alternative Name
- GITR (TNFRSF18 Products)
- Synonyms
- GITR Protein, TNFRSF18 Protein, AITR Protein, Gitr Protein, CD357 Protein, GITR-D Protein, TNF receptor superfamily member 18 Protein, tumor necrosis factor receptor superfamily, member 18 Protein, TNFRSF18 Protein, Tnfrsf18 Protein
- Background
- GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
- Molecular Weight
- 15.87 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
- NCBI Accession
- XP_005545180
- Pathways
- Cancer Immune Checkpoints
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