Glypican 3 Protein (GPC3) (AA 25-559) (His tag)
-
- Target See all Glypican 3 (GPC3) Proteins
- Glypican 3 (GPC3)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 25-559
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Glypican 3 protein is labelled with His tag.
- Purpose
- Human GPC3/Glypican 3 Protein
- Sequence
- Gln25-His559
- Characteristics
- Recombinant Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-His559.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GPC3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- Glypican 3 (GPC3)
- Alternative Name
- GPC3 (GPC3 Products)
- Synonyms
- GPC3 Protein, sgb Protein, dgsx Protein, sdys Protein, sgbs Protein, oci-5 Protein, sgbs1 Protein, DGSX Protein, GTR2-2 Protein, MXR7 Protein, OCI-5 Protein, SDYS Protein, SGB Protein, SGBS Protein, SGBS1 Protein, Glypican-3 Protein, glypican 3 Protein, gpc3 Protein, GPC3 Protein, Gpc3 Protein
- Background
- Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
- Molecular Weight
- 61.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 70-115 kDa based on Tris-Bis PAGE result.
- Pathways
- Glycosaminoglycan Metabolic Process
-