GFRAL Protein (AA 19-351) (His-Avi Tag,Biotin)
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- Target See all GFRAL Proteins
- GFRAL (GDNF Family Receptor alpha Like (GFRAL))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-351
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GFRAL protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human GFRAL/GFR alpha-like Protein
- Sequence
- Ser19-Glu351
- Characteristics
- Recombinant Biotinylated Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser19-Glu351.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GDF15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 0.1μg/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product GFRAL Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- GFRAL (GDNF Family Receptor alpha Like (GFRAL))
- Alternative Name
- GFRAL (GFRAL Products)
- Synonyms
- RGD1565220 Protein, C6orf144 Protein, GRAL Protein, UNQ9356 Protein, bA360D14.1 Protein, AY457637 Protein, Gral Protein, GDNF family receptor alpha like Protein, Gfral Protein, GFRAL Protein
- Background
- GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
- Molecular Weight
- 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result.
- UniProt
- Q6UXV0
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