GARP & Latent TGF beta 1 Complex (AA 20-628) protein (His-Avi Tag,Biotin)

Catalog No. ABIN7274706

Product Details

Target: GARP & Latent TGF beta 1 Complex

Protein Type: Recombinant

Protein Characteristics: AA 20-628

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: His-Avi Tag,Biotin

Purpose: Biotinylated Human GARP & Latent TGF beta 1 Complex Protein

Sequence: His20-Leu628 (GARP) & Leu30-Ser390 (Latent TGF beta 1)

Specificity: Uni-Prot: Q14392 (GARP), P01137 (Latent TGF beta 1)

Characteristics: Recombinant Biotinylated Human GARP & Latent TGF beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-GARP&Latent TGF beta 1 Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.1μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GARP & Latent TGF beta 1 Complex

Background: GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.

Molecular Weight: 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 73-78 kDa (GARP)&13 kDa&42-45 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result.

UniProt: Q14392