GARP & Latent TGF beta 1 Complex (AA 20-628) protein (His-Avi Tag)
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- Target
- GARP & Latent TGF beta 1 Complex
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-628
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His-Avi Tag
- Purpose
- Human GARP & Latent TGF Beta 1 Complex Protein
- Sequence
- His20-Leu628 (GARP) & Leu30-Ser390 (Latent TGF beta 1)
- Specificity
- Uni-Prot: Q14392 (GARP), P01137 (Latent TGF beta 1)
- Characteristics
- Recombinant Human GARP & Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1).
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GARP&Latent TGF Beta 1 Complex at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GARP&Latent TGF Beta 1 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- GARP & Latent TGF beta 1 Complex
- Background
- GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
- Molecular Weight
- 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 72-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result.
- UniProt
- Q14392
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