FCGR1 Protein (AA 25-297) (His tag)
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- Target See all FCGR1 Proteins
- FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 25-297
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FCGR1 protein is labelled with His tag.
- Purpose
- Mouse Fc gamma RI/CD64 Protein
- Sequence
- Glu25-Pro297
- Characteristics
- Recombinant Mouse Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-Pro297.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 87.0 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product FCGR1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))
- Alternative Name
- Fc gamma RI (FCGR1 Products)
- Synonyms
- CD64 Protein, CD64A Protein, FCRI Protein, IGFR1 Protein, AI323638 Protein, AV092959 Protein, FcgammaRI Protein, IGGHAFC Protein, FCGR1A Protein, Fcgr1 Protein, Fcgr1b Protein, Fc fragment of IgG receptor Ia Protein, Fc receptor, IgG, high affinity I Protein, FCGR1A Protein, Fcgr1 Protein, Fcgr1a Protein
- Background
- The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains, these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
- Molecular Weight
- 31.5 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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