FGFR2 beta (AA 152-377) protein (His tag)

Catalog No. ABIN7274647

Product Details

Target: FGFR2 beta

Protein Type: Recombinant

Protein Characteristics: AA 152-377

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: His tag

Purpose: Human FGFR2 beta (IIIc) Protein

Sequence: Arg152-Glu377

Characteristics: Recombinant Human FGFR2 beta (IIIc) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg152-Glu377.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FGFR2 beta

Background: Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha  isoform, or only IgII and IgIII, referred to as the beta  isoform.

Molecular Weight: 26.4 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.