FGFR2 beta (AA 152-378) protein (His tag)
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- Target
- FGFR2 beta
- Protein Type
- Recombinant
- Protein Characteristics
- AA 152-378
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Human FGFR2 beta (IIIb) Protein
- Sequence
- Arg152-Glu378
- Characteristics
- Recombinant Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg152-Glu378.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human FGFR2 beta (IIIb) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR2 lllb Antibody, hFc Tag with the EC50 of 9ng/ml determined by ELISA. The affinity constant of 1.98nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- FGFR2 beta
- Background
- Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform.
- Molecular Weight
- 26.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
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