EGF Protein
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- Target See all EGF Proteins
- EGF (Epidermal Growth Factor (EGF))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Sequence
- Asn971-Arg1023
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human EGF at 2μg/ml (100μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 81.0ng/ml determined by ELISA. The affinity constant of 0.05 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Epidermal Growth Factor (EGF) (Active) protein
EGF Origin: Human Host: Escherichia coli (E. coli) > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
Epidermal Growth Factor (EGF) (AA 971-1023) protein (Fc Tag)EGF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)EGF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)EGF Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (Active) proteinEGF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Epidermal Growth Factor (EGF) (AA 974-1026) protein (His tag)EGF Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)EGF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Epidermal Growth Factor (EGF) proteinEGF Origin: Rat Host: Escherichia coli (E. coli) Recombinant 95,00% SDS
Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (Fc Tag)EGF Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (AA 977-1023) protein (GST tag)EGF Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % SDS, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- EGF (Epidermal Growth Factor (EGF))
- Alternative Name
- EGF (EGF Products)
- Background
- HOMG4, EGF, URG,The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
- Molecular Weight
- 5.6 kDa same as Tris-Bis PAGE result.
- Pathways
- NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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