MPXV E8L (AA 1-275) protein (His tag)

Catalog No. ABIN7274504

Product Details

Target: MPXV E8L

Protein Type: Recombinant

Protein Characteristics: AA 1-275

Origin: Mpox Virus

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: His tag

Purpose: Monkeypox virus E8L Protein

Sequence: Met1-Thr275

Characteristics: Recombinant Monkeypox virus E8L Protein is expressed from E.coli with His tag at the N-Terminus.It contains Met1-Thr275.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MPXV E8L

Alternative Name: E8L

Background: E8L is an important protein that mediates the invasion of monkeypox virus into host cells. In the process of invading and completing replication, E8L acts as a surface-binding protein of mature virion and can bind to chondroitin sulfate on the cell surface, so that the virus can attach to target cells.

Molecular Weight: 32.92 kDa. The protein migrates to 50-60 kDa based on Tris-Bis PAGE result.