SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) (H172Y) Protein
-
- Target See all SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) products
- SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)
- Protein Type
- Recombinant
- Protein Characteristics
- H172Y
- Origin
- SARS Coronavirus-2 (SARS-CoV-2)
-
Source
- Escherichia coli (E. coli)
- Purpose
- SARS-CoV-2 3CLpro/3C-like Protease Protein (H172Y)
- Sequence
- Ser1-Gln306 (H172Y)
- Characteristics
- Recombinant SARS-CoV-2 3CLpro/3C-like Protease Protein (H172Y) is expressed from E.coli without tag.It contains Ser1-Gln306(H172Y).
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
-
-
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- Supplied as 0.22μm filtered solution in 25 mM HEPES, 2.5 mM DTT, 10 % Glycerol ( pH 7.5).
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -80 °C
- Storage Comment
- Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)
- Alternative Name
- 3CLpro (SARS-CoV NSP8 Products)
- Synonyms
- pp1a Protein, pp1ab Protein, orf1ab polyprotein (pp1ab) Protein, orf1ab Protein
- Target Type
- Viral Protein
- Background
- 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase.
- Molecular Weight
- 33.82 kDa same as Tris-Bis PAGE result.
- NCBI Accession
- YP_009725301
-