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SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) (H172Y) Protein

SARS-CoV NSP8 Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7274372
  • Target See all SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) products
    SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)
    Protein Type
    Recombinant
    Protein Characteristics
    H172Y
    Origin
    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 11
    Escherichia coli (E. coli)
    Purpose
    SARS-CoV-2 3CLpro/3C-like Protease Protein (H172Y)
    Sequence
    Ser1-Gln306 (H172Y)
    Characteristics
    Recombinant SARS-CoV-2 3CLpro/3C-like Protease Protein (H172Y) is expressed from E.coli without tag.It contains Ser1-Gln306(H172Y).
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Supplied as 0.22μm filtered solution in 25 mM HEPES, 2.5 mM DTT, 10 % Glycerol ( pH 7.5).
    Preservative
    Dithiothreitol (DTT)
    Precaution of Use
    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -80 °C
    Storage Comment
    Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)
    Alternative Name
    3CLpro (SARS-CoV NSP8 Products)
    Synonyms
    pp1a Protein, pp1ab Protein, orf1ab polyprotein (pp1ab) Protein, orf1ab Protein
    Target Type
    Viral Protein
    Background
    3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase.
    Molecular Weight
    33.82 kDa same as Tris-Bis PAGE result.
    NCBI Accession
    YP_009725301
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