CD40 Ligand Protein (CD40LG) (Trimer) (Fc Tag)
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- Target See all CD40 Ligand (CD40LG) Proteins
- CD40 Ligand (CD40LG)
- Protein Type
- Recombinant
- Protein Characteristics
- Trimer
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD40 Ligand protein is labelled with Fc Tag.
- Purpose
- Mouse CD40 Ligand/TNFSF5 Trimer Protein
- Sequence
- Met112-Leu260
- Characteristics
- Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Met112-Leu260.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 78.3ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product CD40LG Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD40 Ligand (CD40LG)
- Alternative Name
- CD40 Ligand (CD40LG Products)
- Synonyms
- CD154 Protein, cd40l Protein, CD40L Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TNFSF5 Protein, TRAP Protein, gp39 Protein, hCD40L Protein, CD40-L Protein, Cd40l Protein, Ly-62 Protein, Ly62 Protein, Tnfsf5 Protein, CD40 ligand Protein, TNF superfamily member 5 Protein, CD40LG Protein, cd40l Protein, Cd40lg Protein
- Background
- CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
- Molecular Weight
- 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result.
- UniProt
- P27548
- Pathways
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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