FCGR3A Protein (His-Avi Tag,Biotin)
-
- Target See all FCGR3A Proteins
- FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FCGR3A protein is labelled with His-Avi Tag,Biotin.
- Sequence
- Gly17-Gln208(F176)
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA. The affinity constant of 0.14μM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product FCGR3A Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
- Alternative Name
- Fc gamma RIIIA (FCGR3A Products)
- Synonyms
- CD16 Protein, CD16A Protein, FCG3 Protein, FCGR3 Protein, FCGRIII Protein, FCR-10 Protein, FCRIII Protein, FCRIIIA Protein, IGFR3 Protein, Fcgr4 Protein, Fc fragment of IgG receptor IIIa Protein, FCGR3A Protein, Fcgr3a Protein
- Background
- CD16FCRIIIA, Fc fragment of IgG, Fc gamma RIIIA, Fc-gamma RIII-alpha, FCGR3, FCGR3A, FCGRIII, FcgRIIIA, FcR-10, CD16A, FCG3, IGFR3, FcRIIIa, FcRIII, CD16, IMD20,Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
- Molecular Weight
- 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
-