CD300a Protein (CD300A) (AA 18-180) (His tag)
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- Target See all CD300a (CD300A) Proteins
- CD300a (CD300A)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 18-180
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD300a protein is labelled with His tag.
- Purpose
- Human CD300A Protein
- Sequence
- Leu18-Pro180
- Characteristics
- Recombinant Human CD300A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu18-Pro180.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD300A, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 24.2ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD300a (CD300A)
- Alternative Name
- CD300A (CD300A Products)
- Synonyms
- CD300L-B1 Protein, CD300A Protein, clm8 Protein, B230315M08Rik Protein, Clm8 Protein, LMIR1 Protein, MAIR-I Protein, MAIR-Ia Protein, MMAC8 Protein, Pigr4 Protein, mcpir1 Protein, CLM-8 Protein, CMRF-35-H9 Protein, CMRF-35H Protein, CMRF35-H Protein, CMRF35-H9 Protein, CMRF35H Protein, CMRF35H9 Protein, IGSF12 Protein, IRC1 Protein, IRC1/IRC2 Protein, IRC2 Protein, IRp60 Protein, CD300 molecule like family member g Protein, CD300a molecule Protein, CMRF35-like molecule 8 Protein, CD300A molecule Protein, Cd300a molecule Protein, CD300LG Protein, CD300A Protein, LOC483289 Protein, clm8 Protein, Cd300a Protein
- Background
- CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI), however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation.
- Molecular Weight
- 18.7 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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