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CD58 Protein (CD58) (AA 29-215) (Fc Tag)

CD58 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7274195
  • Target See all CD58 Proteins
    CD58
    Protein Type
    Recombinant
    Protein Characteristics
    AA 29-215
    Origin
    Human
    Source
    • 7
    • 3
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This CD58 protein is labelled with Fc Tag.
    Purpose
    Human CD58 Protein
    Sequence
    Phe29-Arg215
    Characteristics
    Recombinant Human CD58 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe29-Arg215.
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    The affinity constant of 9.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    CD58
    Alternative Name
    CD58 (CD58 Products)
    Synonyms
    LFA-3 Protein, LFA3 Protein, ag3 Protein, CD58 molecule Protein, CD58 Protein
    Background
    Loss of CD58 is a common mechanism for tumor immune evasion in lymphoid malignancies. CD58 loss is known to occur due to both genetic and non-genetic causes, therefore, we hypothesized that restoring CD58 expression in lymphoma cells may be an effective treatment approach. EZH2 is involved in the epigenetic silencing of CD58 in lymphoma cells as a mechanism for tumor immune escape, and EZH2 inhibitors are able to restore epigenetically suppressed CD58 expression.
    Molecular Weight
    48.1 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
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