CD4 Protein (CD4) (His-Avi Tag,Biotin)

Catalog No. ABIN7274185

Product Details

Target: CD4

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD4 protein is labelled with His-Avi Tag,Biotin.

Sequence: Lys26-Trp390

Purity: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CD4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Storage: 4 °C,-80 °C

Storage Comment: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD4

Alternative Name: CD4 (CD4 Products)

Synonyms: cd4 Protein, L3T4 Protein, Ly-4 Protein, CD4mut Protein, W3/25 Protein, p55 Protein, CD4 molecule Protein, carcinoembryonic antigen-related cell adhesion molecule 5 Protein, T-cell surface glycoprotein CD4 Protein, CD4 antigen Protein, Cd4 molecule Protein, CD4 Protein, LOC100125525 Protein, cd4 Protein, Cd4 Protein

Background: CD4 antigen (p55), CD4 Molecule, CD4 receptor, CD4, CD4mut, EU3,CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.

Molecular Weight: 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events