CD3D & CD3E protein (His tag)
-
- Target
- CD3D & CD3E
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- His tag
- Sequence
- Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA. The affinity constant of 0.36nM as determined in SPR assay (Biacore T200). See testing image for detail.
-
CD3D & CD3E (AA 23-126) protein (Fc Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 22-117) protein (Fc Tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E protein (Fc-Avi Tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
CD3D & CD3E (AA 22-117) protein (Fc Tag,Biotin)Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 23-120) protein (His tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 23-108) protein (Fc Tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 23-126) (Active) protein (His tag)Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD3D & CD3E (AA 23-126) (Active) protein (His tag,Fc Tag,DYKDDDDK Tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD3D & CD3E (AA 23-126) (Active) protein (Fc Tag,His tag,DYKDDDDK Tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD3D & CD3E (AA 23-126) (Active) protein (His tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- CD3D & CD3E
- Alternative Name
- CD3E&CD3D
- Synonyms
- AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, CD3 antigen, epsilon polypeptide Protein, Cd3e Protein
- Background
- CD3, CD3e, CD3E, CD3d, T3D, CD3D, CD3E&CD3D, CD3 delta&CD3 epsilon,T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
- Molecular Weight
- 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 35-42 kDa and 25 kDa based on Tris-Bis PAGE result.
-
