CD21 Protein (AA 21-971) (His tag)
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- Target See all CD21 (CR2) Proteins
- CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-971
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD21 protein is labelled with His tag.
- Purpose
- Human CD21 Protein
- Sequence
- Ile21-Arg971
- Characteristics
- Recombinant Human CD21 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Arg971.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD21, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD21 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))
- Alternative Name
- CD21 (CR2 Products)
- Synonyms
- C3DR Protein, CD21 Protein, CR Protein, CVID7 Protein, SLEB9 Protein, Cd21 Protein, cd21 Protein, CR1 Protein, CR1-L Protein, CR2 Protein, complement C3d receptor 2 Protein, complement C3b/C4b receptor 1 (Knops blood group) Protein, CR2 Protein, Cr2 Protein, CR1 Protein
- Background
- CD21 mRNA expression, an EBV-entry receptor, was transiently detected in NK cells after exosome treatment, and levels decreased after further culture. CD21 protein expression was also transiently transferred to NK cells after co-culture with an EBV-positive Burkitt lymphoma cell line (Raji) via trogocytosis. However, EBV did not infect NK cells through CD21-mediated trogocytosis.
- Molecular Weight
- 105.3 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.
- Pathways
- Complement System
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