CD14 Protein (CD14) (AA 20-344) (His tag)
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- Target See all CD14 Proteins
- CD14
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-344
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD14 protein is labelled with His tag.
- Purpose
- Human CD14 Protein
- Sequence
- Thr20-Met344
- Characteristics
- Recombinant Human CD14 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Met344.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD14
- Alternative Name
- CD14 (CD14 Products)
- Synonyms
- CD14 molecule Protein, CD14 antigen Protein, CD14 Protein, Cd14 Protein
- Background
- Human monocyte differentiation antigen CD14 is a pattern recognition receptor (PRR) that enhances innate immune responses. CD14 was first identified as a marker of monocytes to signal intracellular responses upon bacterial encounters. Given the absence of an intracellular tail, CD14 was doubted to have the signaling capacities.
- Molecular Weight
- 38.2 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Tris-Bis PAGE result.
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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