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CD14 Protein (CD14) (AA 20-344) (His tag)

CD14 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7274064
  • Target See all CD14 Proteins
    CD14
    Protein Type
    Recombinant
    Protein Characteristics
    AA 20-344
    Origin
    • 17
    • 4
    • 4
    • 1
    • 1
    • 1
    Human
    Source
    • 14
    • 9
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This CD14 protein is labelled with His tag.
    Purpose
    Human CD14 Protein
    Sequence
    Thr20-Met344
    Characteristics
    Recombinant Human CD14 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Met344.
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    CD14
    Alternative Name
    CD14 (CD14 Products)
    Synonyms
    CD14 molecule Protein, CD14 antigen Protein, CD14 Protein, Cd14 Protein
    Background
    Human monocyte differentiation antigen CD14 is a pattern recognition receptor (PRR) that enhances innate immune responses. CD14 was first identified as a marker of monocytes to signal intracellular responses upon bacterial encounters. Given the absence of an intracellular tail, CD14 was doubted to have the signaling capacities.
    Molecular Weight
    38.2 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Tris-Bis PAGE result.
    Pathways
    TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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