C5 Protein (AA 19-1676) (His tag)
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- Target See all C5 Proteins
- C5 (Complement Component 5 (C5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-1676
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This C5 protein is labelled with His tag.
- Purpose
- Human Complement C5 Protein
- Sequence
- Gln19-Cys1676
- Characteristics
- Recombinant Human Complement C5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Cys1676.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Complement C5, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- C5 (Complement Component 5 (C5))
- Alternative Name
- Complement C5 (C5 Products)
- Synonyms
- C5 Protein, LOC565774 Protein, C5a Protein, C5b Protein, CPAMD4 Protein, Hc Protein, RGD1561905 Protein, He Protein, complement C5 Protein, complement component 5 Protein, hemolytic complement Protein, C5 Protein, c5 Protein, Hc Protein
- Background
- Complement component 5 (C5) is an essential factor of the defensive complement system in all vertebrates.C5 plays a major role in complement activation, C5 convertase cleaves C5 into the pro-inflammatory C5a, and C5b, the nidus for the formation of the lytic membrane attack complex.
- Molecular Weight
- 112.5 kDa (α chain) and 73.29 (β chain). Due to glycosylation, the protein migrates to 110-114 kDa (α chain) and 69-73 (β chain) based on Tris-Bis PAGE result.
- Pathways
- Complement System, Carbohydrate Homeostasis
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