EpCAM Protein (AA 24-265) (His-Avi Tag,Biotin)
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- Target See all EpCAM (EPCAM) Proteins
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-265
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This EpCAM protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human EpCAM/TROP1 Protein
- Sequence
- Gln24-Lys265
- Characteristics
- Recombinant Biotinylated Human EpCAM/TROP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Lys265.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Human EpCAM, His Tag at 0.2μg/ml (100ul/Well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- Alternative Name
- EpCAM (EPCAM Products)
- Synonyms
- DIAR5 Protein, EGP-2 Protein, EGP314 Protein, EGP40 Protein, ESA Protein, HNPCC8 Protein, KS1/4 Protein, KSA Protein, M4S1 Protein, MIC18 Protein, MK-1 Protein, TACSTD1 Protein, TROP1 Protein, ECS-1 Protein, ECS1 Protein, ESA1 Protein, M17S1 Protein, EGP Protein, Ep-CAM Protein, sb:cb6 Protein, tacstd Protein, wu:fj17g02 Protein, zgc:110304 Protein, zgc:77119 Protein, tacstd1 Protein, MGC80540 Protein, EPCAM Protein, egp Protein, ksa Protein, m4s1 Protein, mk-1 Protein, cd326 Protein, egp40 Protein, mic18 Protein, trop1 Protein, ep-cam Protein, hegp-2 Protein, co17-1a Protein, ga733-2 Protein, CD326 Protein, Egp314 Protein, EpCAM1 Protein, GA733-2 Protein, Ly74 Protein, Tacsd1 Protein, Tacstd1 Protein, gp40 Protein, epithelial cell adhesion molecule Protein, flotillin 2 Protein, epithelial cell adhesion molecule S homeolog Protein, EPCAM Protein, FLOT2 Protein, epcam Protein, epcam.S Protein, Epcam Protein
- Background
- Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF-like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
- Molecular Weight
- 30.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
- UniProt
- P16422
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