Calprotectin Protein (S100A8/A9) (His tag)

Catalog No. ABIN7274018

Product Details

Target: Calprotectin (S100A8/A9)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Calprotectin protein is labelled with His tag.

Sequence: Met1-Glu93(S100A8) & Thr2-Pro114(S100A9)

Purity: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 2 mM DTT, PBS ( pH 7.2). Normally 5 % trehalose is added as protectant before lyophilization.

Storage: 4 °C,-80 °C

Storage Comment: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Calprotectin (S100A8/A9)

Alternative Name: Calprotectin (S100A8/A9 Products)

Background: Protein S100-A8,Calgranulin-A,CFAG,MRP-8,S100 calcium-binding protein A8,Protein S100-A9,MRP-14,S100 calcium-binding protein A9,Calprotectin, a member of the widespread calcium-binding S-100 protein family, is present in remarkably high concentration in the cytoplasm of human neutrophils. The calprotectin molecule is composed of light (MRP8) and heavy (MRP14) subunits. Although not secreted from intact neutrophils, calprotectin release from dead and dying neutrophils creates high concentrations of the protein in inflammatory or abscess fluids and in the intestinal tract lumen of patients with inflammatory bowel disease.

Molecular Weight: 11.7 kDa(S100A8)&13.2 kDa(S100A9) same as Tris-Bis PAGE result.

Pathways: S100 Proteins