Calprotectin Protein (S100A8/A9) (His tag)
S100A8/A9
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
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- Target See all Calprotectin (S100A8/A9) Proteins
- Calprotectin (S100A8/A9)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Calprotectin protein is labelled with His tag.
- Sequence
- Met1-Glu93(S100A8) & Thr2-Pro114(S100A9)
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Calprotectin (S100A8/A9) (AA 1-119) protein (His tag)
S100A8/A9 Origin: Chicken Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 2 mM DTT, PBS ( pH 7.2). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Calprotectin (S100A8/A9)
- Alternative Name
- Calprotectin (S100A8/A9 Products)
- Synonyms
- 60B8AG Protein, CAGB Protein, CFAG Protein, CGLB Protein, L1AG Protein, LIAG Protein, MAC387 Protein, MIF Protein, MRP14 Protein, NIF Protein, P14 Protein, Mrp14 Protein, MRP-14 Protein, p14 Protein, 60B8Ag Protein, AW546964 Protein, BEE22 Protein, Cagb Protein, GAGB Protein, L1Ag Protein, S100 calcium binding protein A9 Protein, S100 calcium binding protein A9 (calgranulin B) Protein, S100A9 Protein, S100a9 Protein
- Background
- Protein S100-A8,Calgranulin-A,CFAG,MRP-8,S100 calcium-binding protein A8,Protein S100-A9,MRP-14,S100 calcium-binding protein A9,Calprotectin, a member of the widespread calcium-binding S-100 protein family, is present in remarkably high concentration in the cytoplasm of human neutrophils. The calprotectin molecule is composed of light (MRP8) and heavy (MRP14) subunits. Although not secreted from intact neutrophils, calprotectin release from dead and dying neutrophils creates high concentrations of the protein in inflammatory or abscess fluids and in the intestinal tract lumen of patients with inflammatory bowel disease.
- Molecular Weight
- 11.7 kDa(S100A8)&13.2 kDa(S100A9) same as Tris-Bis PAGE result.
- Pathways
- S100 Proteins
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