CA9 Protein (AA 38-414) (His-Avi Tag)
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- Target See all CA9 Proteins
- CA9 (Carbonic Anhydrase IX (CA9))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 38-414
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CA9 protein is labelled with His-Avi Tag.
- Purpose
- Human CA9/Carbonic Anhydrase IX Protein
- Sequence
- Gln38-Asp414
- Characteristics
- Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln38-Asp414.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CA9, His Tag at 0.9μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product CA9 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 50 mM Tris, 150 mM NaCl ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CA9 (Carbonic Anhydrase IX (CA9))
- Alternative Name
- CA9 (CA9 Products)
- Synonyms
- CA9 Protein, CAIX Protein, Ca9 Protein, MN/CA9 Protein, MN Protein, carbonic anhydrase 9 Protein, CA9 Protein, CAH9 Protein, Car9 Protein
- Background
- CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.
- Molecular Weight
- 43.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
- UniProt
- Q16790
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