BTN3A1 Protein (AA 1-219) (His-Avi Tag)
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- Target See all BTN3A1 Proteins
- BTN3A1 (Butyrophilin, Subfamily 3, Member A1 (BTN3A1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-219
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Origin
- Cynomolgus
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This BTN3A1 protein is labelled with His-Avi Tag.
- Purpose
- Cynomolgus BTN3A1/CD277 Protein
- Sequence
- Gln1-Trp219
- Characteristics
- Recombinant Cynomolgus BTN3A1/CD277 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln1-Trp219.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus BTN3A1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product BTN3A1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- BTN3A1 (Butyrophilin, Subfamily 3, Member A1 (BTN3A1))
- Alternative Name
- BTN3A1 (BTN3A1 Products)
- Synonyms
- BT3.1 Protein, BTF5 Protein, CD277 Protein, BTN3A1 Protein, butyrophilin subfamily 3 member A1 Protein, BTN3A1 Protein, LOC100447925 Protein
- Background
- The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
- Molecular Weight
- 26.5 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
- UniProt
- A0A330KWZ1
- Pathways
- Activated T Cell Proliferation
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