Albumin Protein (ALB) (AA 25-609) (His-Avi Tag,Biotin)
-
- Target See all Albumin (ALB) Proteins
- Albumin (ALB)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 25-609
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Albumin protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human Serum Albumin Protein
- Sequence
- Asp25-Leu609
- Characteristics
- Recombinant Biotinylated Human Serum Albumin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp25-Leu609.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 1.67 μM as determined in SPR assay (Biacore T200). See testing image for detail.
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- Albumin (ALB)
- Alternative Name
- Serum Albumin (ALB Products)
- Synonyms
- PRO0883 Protein, PRO0903 Protein, PRO1341 Protein, ALB Protein, CSA Protein, Alb-1 Protein, Alb1 Protein, Albza Protein, LOC100136344 Protein, alb-a Protein, alb-b Protein, albumin Protein, serum albumin 1 Protein, albumin S homeolog Protein, ALB Protein, Alb Protein, LOC100136344 Protein, alb.S Protein
- Background
- Human serum albumin (HSA), the most prominent protein in plasma, binds different classes of ligands at multiple sites. HSA provides a depot for many compounds, affects pharmacokinetics of many drugs, holds some ligands in a strained orientation providing their metabolic modification, renders potential toxins harmless transporting them to disposal sites, accounts for most of the antioxidant capacity of human serum, and acts as a NO-carrier.
- Molecular Weight
- 69.4 kDa. Due to glycosylation, the protein migrates to 69-70 kDa based on Tris-Bis PAGE result.
- Pathways
- Lipid Metabolism
-