VTCN1 Protein (AA 29-258) (Fc Tag)
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- Target See all VTCN1 Proteins
- VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-258
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This VTCN1 protein is labelled with Fc Tag.
- Purpose
- Human B7-H4 Protein
- Sequence
- Phe29-Ala258
- Characteristics
- Recombinant Human B7-H4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe29-Ala258.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human B7-H4, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product VTCN1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
- Alternative Name
- B7-H4 (VTCN1 Products)
- Synonyms
- B7h4 Protein, B7s1 Protein, B7x Protein, BC032925 Protein, B7-H4 Protein, B7H4 Protein, B7S1 Protein, B7X Protein, B7h.5 Protein, PRO1291 Protein, VCTN1 Protein, RGD1311204 Protein, V set domain containing T cell activation inhibitor 1 L homeolog Protein, V-set domain containing T cell activation inhibitor 1 Protein, V-set domain containing T-cell activation inhibitor 1 Protein, V set domain containing T cell activation inhibitor 1 Protein, vtcn1.L Protein, VTCN1 Protein, vtcn1 Protein, Vtcn1 Protein
- Background
- B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status .
- Molecular Weight
- 52.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
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