ICOSLG Protein (AA 19-258) (Fc Tag)
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- Target See all ICOSLG Proteins
- ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-258
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ICOSLG protein is labelled with Fc Tag.
- Purpose
- Human B7-H2/ICOSLG Protein
- Sequence
- Asp19-Ser258
- Characteristics
- Recombinant Human B7-H2/ICOSLG Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp19-Ser258.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human ICOS (C136S,C137S) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 8.9ng/ml determined by ELISA. The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product ICOSLG Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))
- Alternative Name
- B7-H2 (ICOSLG Products)
- Synonyms
- ICOSLG Protein, AU044799 Protein, B7-H2 Protein, B7RP-1 Protein, B7h Protein, BG071784 Protein, GI50 Protein, GL50 Protein, GL50-B Protein, ICOS-L Protein, Icoslg Protein, LICOS Protein, Ly115l Protein, mKIAA0653 Protein, B7H2 Protein, B7RP1 Protein, CD275 Protein, ICOSL Protein, Icosl Protein, RGD1562791 Protein, inducible T-cell costimulator ligand Protein, icos ligand Protein, inducible T-cell co-stimulator ligand Protein, ICOSLG Protein, Icosl Protein, Icoslg Protein
- Background
- B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion, induces also B-cell proliferation and differentiation into plasma cells.
- Molecular Weight
- 53.4 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.
- Pathways
- Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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