APOA1 Protein (AA 25-267) (Fc Tag)
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- Target See all APOA1 Proteins
- APOA1 (Apolipoprotein A-I (APOA1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 25-267
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This APOA1 protein is labelled with Fc Tag.
- Purpose
- Human APOA1 Protein
- Sequence
- Asp25-Gln267
- Characteristics
- Recombinant Human APOA1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp25-Gln267.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- APOA1 (Apolipoprotein A-I (APOA1))
- Alternative Name
- APOA1 (APOA1 Products)
- Synonyms
- Alp-1 Protein, Apoa-1 Protein, Brp-14 Protein, Ltw-1 Protein, Lvtw-1 Protein, Sep-1 Protein, Sep-2 Protein, Sep2 Protein, apoA-I Protein, apoa Protein, apoa1 Protein, cb49 Protein, wu:fb33f01 Protein, zgc:103718 Protein, MGC64335 Protein, MGC89745 Protein, Apo-AI Protein, ApoA-I Protein, APOA1 Protein, LOC100136573 Protein, apoa-i Protein, ApoAI Protein, apolipoprotein A1 Protein, apolipoprotein A-I Protein, apolipoprotein A-Ia Protein, apolipoprotein A-I S homeolog Protein, apolipoprotein A4 Protein, APOA1 Protein, Apoa1 Protein, apoa1a Protein, apoa1.S Protein, apoa1 Protein, LOC100136573 Protein, apoa-i Protein, apoa4 Protein
- Background
- Apolipoprotein A1 (ApoA1) is a main protein moiety in high-density lipoprotein (HDL) particles. Generally, ApoA1 and HDL are considered as atheroprotective. In prooxidant and inflammatory microenvironment in the vicinity to the atherosclerotic lesion, ApoA1/HDL are subjected to modification. The chemical modifications such as oxidation, nitration, etc result in altering native architecture of ApoA1 toward dysfunctionality and abnormality.
- Molecular Weight
- 54.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
- UniProt
- P02647
- Pathways
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
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