MPXV A30L (AA 22-146) protein (His tag)
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- Target
- MPXV A30L
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-146
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Origin
- Mpox Virus
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Monkeypox virus A30 Protein
- Sequence
- Gln22-Leu146
- Characteristics
- Recombinant Monkeypox virus A30 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln22-Leu146.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- MPXV A30L
- Alternative Name
- A30
- Background
- Monkeypox virus (MPXV) is double-stranded DNA virus belonging to the genus orthopoxvirus that causes a smallpox-like disease in humans. A30L is an envelope protein required for the fusion of virus and host cell to form syncytia, and is also considered to be an important target in MPXV research.
- Molecular Weight
- 15.24 kDa. Due to glycosylation, the protein migrates to 30-55 kDa based on Tris-Bis PAGE result.
- UniProt
- Q8V4U9
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