HLA-B*35:01 protein (SBP Tag)
Origin: Human
Host: Mammalian Cells
Recombinant
> 80 %
BP, CP, ELISA, Neut
-
- Target
- HLA-B*35:01
- Protein Type
- Recombinant
- Origin
- Human
- Source
- Mammalian Cells
- Purification tag / Conjugate
- SBP Tag
- Application
- Blocking Peptide (BP), Control Peptide (CP), ELISA, Neutralization (Neut)
- Purpose
- The product is HLA-B*35:01 complexed with β2-microglobulin (b2m)
- Sequence
-
heavy chain: GSHSMRYFYT AMSRPGRGEP RFIAVGYVDD TQFVRFDSDA ASPRTEPRAP WIEQEGPEYW DRNTQIFKTN TQTYRESLRN LRGYYNQSEA GSHIIQRMYG CDLGPDGRLL RGHDQSAYDG KDYIALNEDL SSWTAADTAA QITQRKWEAA RVAEQLRAYL EGLCVEWLRR YLENGKETLQ RADPPKTHVT HHPVSDHEAT LRCWALGFYP AEITLTWQRD GEDQTQDTEL VETRPAGDRT FQKWAAVVVP SGEEQRYTCH VQHEGLPKPL TLRWEPSSQS TIPI
light chain: IQRTPKIQVY SRHPAENGKS NFLNCYVSGF HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC RVNHVTLSQP KIVKWDRDM
Sequences without tags. Tag location is at the discretion of the manufacturer. - Specificity
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HLA-B*35:01 complexed with b2m, P01889 / P61769
HLA-B35 is frequently found in the Caucasoid and the Amerindian major ethnic group, but also in Orientals and Blacks. HLA-B*35:01 is recognized by anti-Bw6 antibodies. - Characteristics
- HLAs are produced in mammalian cell cultures as recombinant, truncated, soluble antigens, in complex with β2-microglobulin (HLA class I). As part of our quality control, all HLA are analyzed for purity by gel electrophoresis, concentration is determined by ELISA (0.5 mg/ml) and their functionality is verified with a specific antiserum.
- Purification
- Chromatography
- Purity
- > 80 %
- Endotoxin Level
- Endotoxins have not been removed
-
- Application Notes
- ELISA: 1 - 100 ng/mL
- Comment
-
sandwich ELISA, direct ELISA, bead ELISA, reactive with antibodies W6/32 and BBM.1 and specific antiserum, B cell ELI Spot (predicted), in vitro memory B cell stimulation (predicted), HLA competition assay (predicted), control in various assays (predicted)
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- TRIS based buffer, pH 8.0, Sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Expiry Date
- 6 months
-
- Target
- HLA-B*35:01
- Background
- Human Leukozyte Antigen, human Antigen-presenting major histocompatibility complex class I (MHCI) molecule. Expressed on all cells, displays peptides to CD8-positive T cells. Involved in cellular immune response.
- Molecular Weight
- approx. 50 kDa (38 kDa heavy chain + 12 kDa b2m)
- UniProt
- P01889
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