IDH1 Protein (Arg132Cys-Mutant) (His tag)
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- Target See all IDH1 Proteins
- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
- Protein Type
- Recombinant
- Protein Characteristics
- Arg132Cys-Mutant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IDH1 protein is labelled with His tag.
- Purpose
- Human IDH1 (R132C) Protein, His Tag (MALS verified)
- Sequence
- Met 1 - Leu 414
- Characteristics
- Human IDH1 (R132C), His Tag (ID1-H51H8) is expressed from E.coli cells. It contains AA Met 1 - Leu 414 (Accession # O75874-1(R132C).
- Purity
- >95 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Grade
- MALS verified
- Top Product
- Discover our top product IDH1 Protein
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Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 2-414) protein (His tag)
IDH1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 341-414) protein (His-IF2DI Tag)IDH1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 1-414) protein (His tag)IDH1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 1-414) protein (GST tag)IDH1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Application Notes
- This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 48.5 kDa. The protein migrates as 47-50 kDa under reducing (R) condition due to glycosylation.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH 7.5
- Storage
- -80 °C
- Storage Comment
- -70°C
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- Target
- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
- Alternative Name
- IDH1 (IDH1 Products)
- Synonyms
- IDCD Protein, IDH Protein, IDP Protein, IDPC Protein, PICD Protein, NADP-CICDH Protein, AI314845 Protein, AI788952 Protein, E030024J03Rik Protein, Id-1 Protein, Idh-1 Protein, Idpc Protein, cb876 Protein, fm90e09 Protein, im:7143416 Protein, wu:fm90e09 Protein, F23E12.180 Protein, F23E12_180 Protein, IDH-I Protein, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1 Protein, isocitrate dehydrogenase 1 Protein, isocitrate dehydrogenase I Protein, isocitrate dehydrogenase (NADP(+)) 1, cytosolic Protein, isocitrate dehydrogenase 1 (NADP+), soluble Protein, isocitrate dehydrogenase 1 (NADP+) L homeolog Protein, isocitrate dehydrogenase 1 Protein, IDH1 Protein, Idh1 Protein, idh1.L Protein, idh1 Protein
- Background
- Synonyms:IDH1,PICD,IDP,Description:The IDH enzymes catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (α-KG), producing nicotinamide adenine dinucleotide phosphate (NADPH) in the process via the citric acid cycle. Eukaryotic cells express two distinct classes of IDHs that utilize either NAD or NADP as their cofactors and serve diverse biological functions. NAD-dependent IDH is localized to the mitochondrial matrix and is well known for its central role for energy production in the Krebs cycle. NADP-dependent IDHs are primarily located either in mitochondria or cytoplasm . Each NADP-dependent isozyme is a homodimer. Mutations of Arg132 of human IDH1 result in a reduced ability of the enzyme to convert isocitrate to alpha -ketoglutarate, but the enzyme acquires the ability to generate 2-hydroxyglutarate (2HG) from alpha-ketoglutarate,2-HG is elevated in several tumor types, including a subset of AMLs.
- Molecular Weight
- 48.5 kDa
- Pathways
- Warburg Effect
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