NPR1 Protein (His-Avi Tag,Biotin)

Catalog No. ABIN7273415

Product Details

Target: NPR1 (Natriuretic Peptide Receptor A/guanylate Cyclase A (Atrionatriuretic Peptide Receptor A) (NPR1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This NPR1 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human NPR1 / NPRA Protein, His,Avitag™ (MALS verified)

Sequence: Gly 33 - Glu 473

Characteristics: Biotinylated Human NPR1, His,Avitag (NP1-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gly 33 - Glu 473 (Accession # P16066-1).

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Grade: MALS verified

Application Details

Application Notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 52.5 kDa. The protein migrates as 63-70 kDa under reducing (R) condition due to glycosylation.

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: NPR1 (Natriuretic Peptide Receptor A/guanylate Cyclase A (Atrionatriuretic Peptide Receptor A) (NPR1))

Alternative Name: NPR1 / NPRA (NPR1 Products)

Synonyms: ANPRA Protein, ANPa Protein, GUC2A Protein, GUCY2A Protein, NPRA Protein, Anpra Protein, Gca Protein, NPR-A Protein, Npra Protein, sGC Protein, si:dkey-27c15.1 Protein, AI893888 Protein, GC-A Protein, Pndr Protein, ARABIDOPSIS NONEXPRESSER OF PR GENES 1 Protein, ATNPR1 Protein, F15H21.6 Protein, F15H21_6 Protein, NIM1 Protein, NON-INDUCIBLE IMMUNITY 1 Protein, NONEXPRESSER OF PR GENES 1 Protein, REGULATORY PROTEIN NPR1 Protein, SAI1 Protein, SALICYLIC ACID INSENSITIVE 1 Protein, natriuretic peptide receptor 1 Protein, natriuretic peptide receptor 1a Protein, regulatory protein (NPR1) Protein, NPR1 Protein, Npr1 Protein, npr1a Protein

Background: Synonyms:Atrial natriuretic peptide receptor 1,NPR1,NPRA,GC-A,ANPRA,NPR-A,Description:Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.

Molecular Weight: 52.5 kDa

NCBI Accession: NP_000897