VTCN1 Protein (Biotin)

Catalog No. ABIN7319876

This Recombinant VTCN1 protein is expressed in Human Cells.

Quick Overview for VTCN1 Protein (Biotin) (ABIN7319876)

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purity: >95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This VTCN1 protein is labelled with Biotin.

Purpose: Recombinant Human B7 Homolog 4/B7-H4/VTCN1 (C-Fc-Avi) Biotinylated

Sequence: Phe29-Ala258

Characteristics: Biotinylated Recombinant Human B7 Homolog 4 is produced by our Mammalian expression system and the target gene encoding Phe29-Ala258 is expressed with a Fc, Avi tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Anti-Human B7-H4 mAb(Cat#NC054) at 2μg/ml (100 μl/well) can bind Biotinylated Human B7-H4-Fc-Avi(Cat#CY37). The ED50 of Biotinylated Human B7-H4-Fc-Avi(Cat#CY37) is 0.23 ng/ml.

Application Details

Comment:

70-95 kDa

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Alternative Name: VTCN1

Background:

Background: B7 Homolog 4 (B7-H4) is glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H4 consists of a 235 amino acid (aa) extracellular domain (ECD) with two Ig-like V-type domains, a 21 aa transmembrane segment, and a 2 aa cytoplasmic tail. It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.

Synonym: B7S1, B7x, Vtcn1, B7h.5, B7-H4, B7H4T-cell costimulatory molecule B7x, B7S1VCTN1, B7XPRO1291, FLJ22418, Immune costimulatory protein B7-H4, Protein B7S1, T cell costimulatory molecule B7x, V-set domain containing T cell activation inhibitor 1, V-set domain-containing T-cell activation inhibitor 1

Molecular Weight: 54.1 kDa

UniProt: Q7Z7D3