AMPK (A2/B1/G1) (Active) Protein
Origin: Human
Host: Baculovirus infected Insect Cells
Recombinant
> 94 % as determined by reducing SDS-PAGE.
Active
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- Target
- AMPK (A2/B1/G1)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purpose
- Recombinant Human AMPK (G1/B1/A2) Heterotrimer Protein (Active)
- Sequence
- Met 1-Pro 331
- Characteristics
- A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1, A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2, A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
- Purity
- > 94 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
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- Comment
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40, 42 & 95 kDa
- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 50 mM Tris, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.5 mM PMSF, 10 % gly, 1 mM GSH, pH 7.4
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- AMPK (A2/B1/G1)
- Background
- AMPK,AMPKa1
- Molecular Weight
- 158 (38+30+90) kDa
- UniProt
- P54619
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