FGF8 Protein

Catalog No. ABIN7198878

Product Details

Target: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human FGF8a/FGF-8a Protein (Active)

Sequence: Gln23-Arg204

Characteristics: A DNA sequence encoding the human FGF8a (NP_149355.1) (Gln23-Arg204) was expressed with an initial Met.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: Please contact us for more information.

Biological Activity Comment: Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.8-3.3 μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Alternative Name: FGF8a (FGF8 Products)

Synonyms: Aigf Protein, Fgf-8 Protein, AIGF Protein, FGF-8 Protein, HBGF-8 Protein, HH6 Protein, KAL6 Protein, fibroblast growth factor 8 Protein, Fgf8 Protein, FGF8 Protein

Background:

Background: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants.

Synonym: AIGF,FGF-8,HBGF-8,HH6,KAL6

Molecular Weight: 21.3 kDa

NCBI Accession: NP_149355

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis