CD24 Protein (Biotin)

Catalog No. ABIN7319889

Product Details

Target: CD24 (CD24 Molecule (CD24))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This CD24 protein is labelled with Biotin.

Purpose: Recombinant Human Signal Transducer CD24/CD24 (C-Fc-Avi) Biotinylated

Sequence: Ser27-Gly59

Characteristics: Biotinylated Recombinant Human Signal Transducer CD24 is produced by our Mammalian expression system and the target gene encoding Ser27-Gly59 is expressed with a Fc, Avi tag at the C-terminus.

Purity: >95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Anti-Human CD24 mAb(Cat#NC050) at 2μg/ml (100 μl/well) can bind Biotinylated Human CD24-Fc-Avi(Cat#CY43). The ED50 of Biotinylated Human CD24-Fc-Avi(Cat#CY43) is 1.83 ng/ml.

Application Details

Comment:

40-55 kDa

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD24 (CD24 Molecule (CD24))

Alternative Name: CD24 (CD24 Products)

Synonyms: CD24A Protein, Cd24a Protein, CD24 molecule Protein, CD24 Protein, Cd24 Protein

Background:

Background: Signal Transducer CD24 is a heavily and variably glycosylated GPI-linked sialoprotein. Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells. CD24 expression is regulated during lineage development and with the activation of various cell types. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 Molecules in the costimulation of T cells. CD24 associates in cis with Siglec10 and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells. Formation of these ternary complexes fills a protective role: the resulting Siglec10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs.

Synonym: Signal transducer CD24, Small cell lung carcinoma cluster 4 antigen, CD24, CD24A

Molecular Weight: 31.6 kDa

UniProt: P25063

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation