ZIKV E Protein (Fc Tag)
-
- Target See all ZIKV E products
- ZIKV E (Zika Virus Envelope (ZIKV E))
- Protein Type
- Recombinant
- Origin
- Zika Virus (ZIKV)
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ZIKV E protein is labelled with Fc Tag.
- Purpose
- Recombinant ZIKV (strain Zika SPH2015) Envelope protein (Domain III, Fc Tag)
- Sequence
- Val593-Lys699
- Characteristics
- A DNA sequence encoding the Zika virus (strain Zika SPH2015) E / Envelope (domain III) (ALU33341.1) (Val593-Lys699) was expressed with the Fc region of human IgG1 at the C-terminus.
- Purity
- > 90 % as determined by SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg protein as determined by the LAL method.
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- Target
- ZIKV E (Zika Virus Envelope (ZIKV E))
- Alternative Name
- Zika Envelope (ZIKV E Products)
- Background
-
Background: Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76 % of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
Synonym: Zika virus, E protein, strain Zika SPH2015
- Molecular Weight
- 38.2kDa.
-