Vinculin Protein (VCL) (His tag)

Catalog No. ABIN7198696

Product Details

Target: Vinculin (VCL)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This Vinculin protein is labelled with His tag.

Purpose: Recombinant Mouse VCL/Vinculin Protein (His Tag)

Sequence: Met1-Gln1066

Characteristics: A DNA sequence encoding the mouse VCL (NP_033528.3) (Met1-Gln1066) was expressed with a C-terminal polyhistidine tag.

Purity: > 90 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Vinculin (VCL)

Alternative Name: VCL/Vinculin (VCL Products)

Synonyms: CMD1W Protein, CMH15 Protein, MV Protein, MVCL Protein, VCL Protein, im:6902790 Protein, hm:zehn2160 Protein, DDBDRAFT_0186762 Protein, DDBDRAFT_0232320 Protein, DDB_0186762 Protein, DDB_0232320 Protein, 9430097D22 Protein, AA571387 Protein, AI462105 Protein, AW545629 Protein, CG3299 Protein, CT11081 Protein, Dmel\\CG3299 Protein, Dmvincp Protein, EG:103B4.1 Protein, Vin Protein, Vin2EF Protein, vinc Protein, LOC100228062 Protein, vcl-A Protein, vinculin Protein, vinculin a Protein, vinculin A Protein, Vinculin Protein, vinculin S homeolog Protein, VCL Protein, vcla Protein, vinA Protein, Vcl Protein, Vinc Protein, CpipJ_CPIJ013873 Protein, Bm1_37935 Protein, vcl Protein, vcl.S Protein

Background:

Background: Vinculin (VCL) is a cytoskeletal protein that is closely related to both cell-matrix interactions and cell-cell junctions. VCL is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. This protein has multi-ligand properties and has been found to interact with a number of microfilament associated proteins, such as talin, a-actinin, and paxillin, which reportedly bind to either the head or tail domains of vinculin.

Synonym: 9430097D22,AA571387,AI462105,AW545629

Molecular Weight: 118.2 kDa

NCBI Accession: NP_033528

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2