GALE Protein (His tag)
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- Target See all GALE Proteins
- GALE (UDP-Galactose-4-Epimerase (GALE))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GALE protein is labelled with His tag.
- Purpose
- Recombinant Human GALE Protein (His Tag)
- Sequence
- Met 1-Ala348
- Characteristics
- Recombinant Human UDP-Glucose 4-Epimerase is produced by our E.coli expression system and the target gene encoding Met1-Ala348 is expressed with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product GALE Protein
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UDP-Galactose-4-Epimerase (GALE) (AA 1-348) protein (His tag)
GALE Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
UDP-Galactose-4-Epimerase (GALE) (AA 1-348) protein (GST tag)GALE Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
UDP-Galactose-4-Epimerase (GALE) (AA 1-348), (full length) protein (His-SUMO Tag)GALE Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
UDP-Galactose-4-Epimerase (GALE) (Transcript Variant 1) protein (His tag)GALE Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
UDP-Galactose-4-Epimerase (GALE) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GALE Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
UDP-Galactose-4-Epimerase (GALE) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)GALE Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
UDP-Galactose-4-Epimerase (GALE) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)GALE Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM TrisHCl, 150 mM NaCl, 2 mM DTT, 1 mM EDTA, pH 8.0.
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- GALE (UDP-Galactose-4-Epimerase (GALE))
- Alternative Name
- GALE (GALE Products)
- Background
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Background: The enzyme UDP-Glucose 4-Epimerase (GALE) is a homodimeric epimerase found in bacterial, plant and mammalian cells. UDP-Glucose 4-Epimerase performs the final step in the Leloir pathway of Galactose metabolism, it catalyzes two distinct but analogous reactions: the epimerization of UDP-Gglucose to UDP-Galactose and the epimerization of UDP-N-Acetylglucosamine to UDP-N-Acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.
Synonym: UDP-Glucose 4-Epimerase, Galactowaldenase, UDP-Galactose 4-Epimerase, GALE
- Molecular Weight
- 40.4 kDa
- UniProt
- Q14376
- Pathways
- Response to Water Deprivation, Cellular Glucan Metabolic Process
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