UBE2H Protein

Catalog No. ABIN7198586

Product Details

Target: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human Ube2H Protein

Sequence: Met 1-Leu 183

Characteristics: A DNA sequence encoding the human UBE2H (P62256) (Met 1-Leu 183) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.

Purity: > 93 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, 10 % glycerol, 2 mM DTT, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Alternative Name: Ube2H (UBE2H Products)

Synonyms: fc07a05 Protein, fi24c01 Protein, wu:fc07a05 Protein, wu:fi24c01 Protein, si:bz3c13.2 Protein, e2-20k Protein, ubc8 Protein, ubch Protein, ubch2 Protein, E2-20K Protein, GID3 Protein, UBC8 Protein, UBCH Protein, UBCH2 Protein, 1500009C23Rik Protein, AI181839 Protein, AI326965 Protein, AI462483 Protein, AW227540 Protein, Gid3 Protein, N28148 Protein, Ubc8 Protein, ubiquitin conjugating enzyme E2 H Protein, ubiquitin-conjugating enzyme E2 H Protein, Ubiquitin-conjugating enzyme E2 H Protein, ubiquitin conjugating enzyme E2H S homeolog Protein, ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) Protein, ubiquitin-conjugating enzyme E2-21 KD Protein, ubiquitin-conjugating enzyme E2H Protein, UBE2H Protein, CpipJ_CPIJ015105 Protein, Bm1_31760 Protein, PTRG_01621 Protein, LOAG_08716 Protein, ube2h.S Protein, ube2h Protein, uce-E2 Protein, uceE2 Protein, Ube2h Protein

Background:

Background: UBE2H is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1); which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. 

Synonym: Ubiquitin-Conjugating Enzyme E2 H; UbcH2; Ubiquitin Carrier Protein H; Ubiquitin-Conjugating Enzyme E2-20K; Ubiquitin-Protein Ligase H; UBE2H;E2-20K;GID3;UBC8;UBCH;UBCH2

UniProt: P62256