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Tyrosine Hydroxylase Protein (TH) (His tag)

TH Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 94 % as determined by reducing SDS-PAGE.
Catalog No. ABIN7317803
  • Target See all Tyrosine Hydroxylase (TH) Proteins
    Tyrosine Hydroxylase (TH)
    Protein Type
    Recombinant
    Origin
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Source
    • 6
    • 4
    • 1
    • 1
    Baculovirus infected Insect Cells
    Purification tag / Conjugate
    This Tyrosine Hydroxylase protein is labelled with His tag.
    Purpose
    Recombinant Human TH/Tyrosine Hydroxylase Protein (His Tag)
    Sequence
    Pro 2-Gly 497
    Characteristics
    A DNA sequence encoding the human TH isoform 2 (P07101-3) (Pro 2-Gly 497) was fused with a polyhistidine tag at the N-terminus.
    Purity
    > 94 % as determined by reducing SDS-PAGE.
    Endotoxin Level
    < 1.0 EU per μg as determined by the LAL method.
    Top Product
    Discover our top product TH Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Please refer to the printed manual for detailed information.
    Buffer
    Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target
    Tyrosine Hydroxylase (TH)
    Alternative Name
    TH/Tyrosine Hydroxylase (TH Products)
    Synonyms
    DYT14 Protein, DYT5b Protein, TYH Protein, The Protein, CG10118 Protein, DH65B Protein, DTH Protein, Dmel\\CG10118 Protein, Pale Protein, Ple Protein, TH Protein, Th Protein, VII Protein, dTH1 Protein, dTH65B Protein, pale/ple Protein, th Protein, dyt14 Protein, dyt5b Protein, tyh Protein, tyrosine hydroxylase Protein, pale Protein, tyrosine 3-monooxygenase Protein, tyrosine hydroxylase S homeolog Protein, TH Protein, Th Protein, ple Protein, th Protein, th.S Protein, LOC100008895 Protein
    Background

    Background: Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.

    Synonym: DYT14,DYT5b,TYH

    Molecular Weight
    57.6 kDa
    Pathways
    Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
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