TNFRSF10A Protein (His tag)
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- Target See all TNFRSF10A Proteins
- TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TNFRSF10A protein is labelled with His tag.
- Purpose
- Recombinant Human TRAILR1/TNFRSF10A Protein (His Tag)(Active)
- Sequence
- Met 1-Asn 239
- Characteristics
- A DNA sequence encoding the human TNFRSF10A (NP_003835.2) extracellular domain (Met 1-Asn 239) was expressed, fused with a polyhistidine tag at the C-terminus.
- Purity
- > 92 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL.The ED50 for this effect is typically 50-200 ng/ml in the presence of 20 ng/ml Recombinant Human TRAIL/TNFSF10.
- Top Product
- Discover our top product TNFRSF10A Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
- Alternative Name
- TRAILR1/TNFRSF10A (TNFRSF10A Products)
- Synonyms
- APO2 Protein, CD261 Protein, DR4 Protein, TRAILR-1 Protein, TRAILR1 Protein, TNFRSF10A Protein, TNF receptor superfamily member 10a Protein, tumor necrosis factor receptor superfamily member 26 Protein, tumor necrosis factor receptor superfamily, member 10a Protein, TNFRSF10A Protein, LOC510798 Protein
- Background
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Background: Tumor necrosis factor receptor superfamily, member 10a (TRAIL R1), also known as TRAIL receptor 1 (TRAIL R1) or CD261 antigen, is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R1/CD261/TNFRSF10A serves as a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAIL R1 can promote the activation of NF-kappa-B. TRAIL R1/CD261/TNFRSF10A induces apoptosis of many transformed cell lines but not of normal tissues, even though its death domain-containing receptor, DR4, is expressed on both cell types.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Synonym: APO2;CD261;DR4;MGC9365;TNFRSF10A;TRAILR-1;TRAILR1
- Molecular Weight
- 15.7 kDa
- NCBI Accession
- NP_003835
- Pathways
- Apoptosis, Positive Regulation of Endopeptidase Activity
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