Transferrin Protein (TF) (His tag)

Catalog No. ABIN7321071

Product Details

Target: Transferrin (TF)

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This Transferrin protein is labelled with His tag.

Purpose: Recombinant Rat Transferrin/TF Protein (His Tag)(Active)

Sequence: Met1-Ser698

Characteristics: A DNA sequence encoding the rat TF (NP_001013128.1) (Met1-Ser698) was expressed with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect is typically 0.02-1 μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.2

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Transferrin (TF)

Alternative Name: Transferrin/TF (TF Products)

Synonyms: ltf Protein, pro1557 Protein, pro2086 Protein, mgc107777 Protein, LOC692564 Protein, TF Protein, LOC100144362 Protein, tf Protein, LTF Protein, TFEW Protein, conalbumin Protein, tf-b Protein, MGC64306 Protein, PRO1557 Protein, PRO2086 Protein, TFQTL1 Protein, AI266983 Protein, Cd176 Protein, HP Protein, Tf Protein, Tfn Protein, hpx Protein, Trf Protein, cb285 Protein, gavi Protein, id:ibd3238 Protein, id:ibd3525 Protein, sb:cb285 Protein, wu:fb57g06 Protein, wu:fb62h02 Protein, wu:fb63h10 Protein, wu:fb64h10 Protein, zgc:112154 Protein, 143958_at Protein, CG6186 Protein, Dmel\\CG6186 Protein, TSF1 Protein, anon-EST:Posey265 Protein, tsf1 Protein, Pro-TRH Protein, IL-5 Protein, STF I Protein, TRF1 Protein, sTF1 Protein, sTf Protein, tf1 Protein, transferrin Protein, serotransferrin Protein, transferrin (ovotransferrin) Protein, transferrin L homeolog Protein, melanotransferrin Protein, transferrin-a Protein, Transferrin 1 Protein, thyrotropin releasing hormone Protein, interleukin 5 Protein, TF Protein, LOC477072 Protein, tf Protein, Tf Protein, LOC100144362 Protein, tf.L Protein, LOC5575625 Protein, Trf Protein, tfa Protein, Tsf1 Protein, TRH Protein, IL5 Protein, LOC101085148 Protein, LOC100726872 Protein, trf Protein

Background:

Background: Transferrin is a glycoprotein with an approximate molecular weight of 76.5 kDa. This glycoprotein is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of Transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte / pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. Transferrins are iron binding transport proteins which bind Fe3+ ion in association with the binding of an anion, usually bicarbonate. This transferrin binds only one Fe3+ ion per protein molecule. Transports iron ions from the hemolymph into the eggs during the vitellogenic stage. Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. When a transferrin loaded with iron encounters with a transferring receptor on cell surface, transferring binds to it and, as a consequence, is transported into the cell in a visicle by receptor-mediated endocytosis. The PH is reduced by hydrogen iron pumps. The lower pH causes transferrin to release its iron ions. The receptor is then transported through the endocytic cycle back to the cell surface, ready for another round of iron uptake. Each transferrin molecule has the ability to carry two iron ions in the ferric form.

Synonym: TF

Molecular Weight: 76 kDa

NCBI Accession: NP_001013128

Pathways: Transition Metal Ion Homeostasis