TCN2 Protein (His tag)

Catalog No. ABIN7317824

Product Details

Target: TCN2 (Transcobalamin II (TCN2))

Protein Type: Recombinant

Origin: Human

Source: CHO Cells

Purification tag / Conjugate: This TCN2 protein is labelled with His tag.

Purpose: Recombinant Human TCN2 Protein (His Tag)

Sequence: Met 1-Trp 427

Characteristics: A DNA sequence encoding the human TCN2 (NP_000346.2) (Met 1-Trp 427) was expressed with a C-terminal polyhistidine tag.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TCN2 (Transcobalamin II (TCN2))

Alternative Name: TCN2 (TCN2 Products)

Synonyms: AW208754 Protein, Tcn-2 Protein, Tc2 Protein, Tcn2p Protein, D22S676 Protein, D22S750 Protein, II Protein, TC Protein, TC II Protein, TC-2 Protein, TC2 Protein, TCII Protein, transcobalamin 2 Protein, Tcn2 Protein, TCN2 Protein

Background:

Background: Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl, vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.

Synonym: Transcobalamin-II,D22S676,D22S750,II,TC,TC-2,TC2,TCII

Molecular Weight: 46.7 kDa

NCBI Accession: NP_000346