TXN Protein
-
- Target See all TXN Proteins
- TXN (Thioredoxin (TXN))
- Protein Type
- Recombinant
- Biological Activity
- Active
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purpose
- Recombinant Human Thioredoxin/TXN Protein (Active)
- Sequence
- Met 1-Val 105
- Characteristics
- A DNA sequence encoding the C-terminal segment of human TXN (P10599) (Met 1-Val 105) was expressed and purified.
- Purity
- > 97 % as determined by reducing SDS-PAGE.
- Biological Activity Comment
- Measured by its ability to catalyze the reduction of insulin. The specific activity is 5-9 pmoles/min/μg.2. Measured by its ability to catalyze the reduction of insulin. The reaction leads toprecipitation, which can be measured by absorbance at 650 nm. The specific activity is 5-10 A650/min/mg.
- Top Product
- Discover our top product TXN Protein
-
Thioredoxin (TXN) protein
TXN Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Thioredoxin (TXN) (AA 2-109) protein (His tag)TXN Origin: E. coli Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS-PAGE SDS
Thioredoxin (TXN) (AA 1-105) protein (GST tag)TXN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Thioredoxin (TXN) (AA 2-105), (full length) protein (GST tag)TXN Origin: Human Host: Escherichia coli (E. coli) Recombinant 95 % SDS, WB, ELISA
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.5
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- Target
- TXN (Thioredoxin (TXN))
- Alternative Name
- Thioredoxin/TXN (TXN Products)
- Background
-
Background: Thioredoxin; also known as ATL-derived factor; Surface-associated sulphydryl protein; SASP and TXN; is a nucleus; cytoplasm and secreted protein which belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins; and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO); and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
Synonym: Thioredoxin; Trx; ATL-Derived Factor; ADF; Surface-Associated Sulphydryl Protein; SASP; TXN; TRDX; TRX; TRX1
- Molecular Weight
- 11.7 kDa
- UniProt
- P10599
- Pathways
- Carbohydrate Homeostasis, Cell RedoxHomeostasis
-
