SEMA5A Protein (AA 1-968) (His tag)

Catalog No. ABIN7317589

Product Details

Target: SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))

Protein Type: Recombinant

Protein Characteristics: AA 1-968

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SEMA5A protein is labelled with His tag.

Purpose: Recombinant Human Semaphorin 5A/SEMA5A Protein (aa 1-968, His Tag)

Sequence: Met 1-Met 968

Characteristics: A DNA sequence encoding the human SEMA5A (NP_003957.2) extracellular domain (Met 1-Met 968) was fused with a polyhistidine tag at the C-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))

Alternative Name: Semaphorin 5A/SEMA5A (SEMA5A Products)

Synonyms: wu:fa95e03 Protein, wu:fi41a04 Protein, SEMAF Protein, semF Protein, 5930434A13 Protein, 9130201M22Rik Protein, AI464145 Protein, Semaf Protein, semaphorin 5A Protein, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A Protein, semaphorin-5A Protein, SEMA5A Protein, sema5a Protein, Sema5a Protein, LOC100516117 Protein

Background:

Background: Semaphorins are secreted, transmembrane, and GPI-linked proteins, defined by cysteine-rich semaphorin protein domains, that have important roles in a variety of tissues. Humans have 20 semaphorins, Drosophila has five, and two are known from DNA viruses. Semaphorins are found in nematodes and crustaceans but not in non-animals. They are grouped into eight classes on the basis of phylogenetic tree analyses and the presence of additional protein motifs. Semaphorins have been implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration. Semaphorin-5A, also known as Semaphorin-F, Sema F, SEMA5A and SEMAF, is a single-pass type I membrane protein which belongs to the semaphorin family. Semaphorin5A / SEMA5A contains one PSI domain, one Sema domain and seven TSP type-1 domains. It may act as positive axonal guidance cues. Semaphorin5A / SEMA5A is an axon regulator molecule and plays major roles during neuronal and vascular development. It plays an essential role in embryonic development. Semaphorin5A / SEMA5A induces endothelial cell migration from pre-existing vessels. It also plays a role in autism, reducing the ability of neurons to form connections with other neurons in certain brain regions.

Synonym: Semaphorin-5A; Semaphorin-F; Sema F; SEMA5A; SEMAF

Molecular Weight: 107 kDa

NCBI Accession: NP_003957