SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (Biotin,His-Avi Tag)

Catalog No. ABIN7197791

Product Details

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SARS-CoV-2 Nucleocapsid protein is labelled with Biotin,His-Avi Tag.

Purpose: Recombinant SARS-CoV-2 N Protein biotinylated(N-6His-Avi)(Active)

Sequence: Met1-Ala419

Specificity: N-6His-Avi

Characteristics: Biotinylated 2019-nCoV Nucleocapsid Protein is produced by our E.coli expression system and the target gene encoding Met1-Ala419 is expressed with a 6His, Avi tag at the N-terminus.

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Immobilized 2019-nCoV NP Antibody (6G9)at 5μg/ml (100 μl/well) can bind 2019-nCoV Nucleocapsid Protein-His-Avi(PKSV030311). The ED50 of PKSV030311 is 92 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 50 mM Arginine, pH 7.5

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 N (SARS-CoV-2 N Products)

Synonyms: nucleocapsid phosphoprotein Protein, N Protein

Target Type: Viral Protein

Background:

Background: Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Synonym: SARS-CoV-2 coronavirus NP Protein, SARS-CoV-2 np Protein, SARS-CoV-2 novel coronavirus

Molecular Weight: 51.3kDa