RPE Protein (His tag)

Catalog No. ABIN7317450

Product Details

Target: RPE (Ribulose-5-Phosphate-3-Epimerase (RPE))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This RPE protein is labelled with His tag.

Purpose: Recombinant Human RPE Protein (His Tag)

Sequence: Ala 2-Arg 228

Characteristics: A DNA sequence encoding the human RPE isoform 1 (NP_954699.1) (Ala 2-Arg 228) was expressed, with a polyhistidine tag at the N-terminus.

Purity: > 94 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: RPE (Ribulose-5-Phosphate-3-Epimerase (RPE))

Alternative Name: RPE (RPE Products)

Synonyms: wu:fa07h08 Protein, wu:fb93d11 Protein, wu:fc20b11 Protein, ik:tdsubc_2c8 Protein, xx:tdsubc_2c8 Protein, RPE2-1 Protein, 2810429B02Rik Protein, 5730518J08Rik Protein, ribulose-5-phosphate-3-epimerase Protein, rpe Protein, RPE Protein, Rpe Protein

Background:

Background: The "ribulose phosphate binding" superfamily defined by the Structural Classification of Proteins (SCOP) database is considered the result of divergent evolution from a common (beta/alpha)(8)-barrel ancestor. The superfamily includes d-ribulose 5-phosphate 3-epimerase (RPE), orotidine 5'-monophosphate decarboxylase (OMPDC), and 3-keto-l-gulonate 6-phosphate decarboxylase (KGPDC). Replication of the human genome requires the activation of thousands of replicons distributed along each one of the chromosomes. Each replicon contains an initiation, or origin, site, at which DNA synthesis begins. In enzymology, a L-ribulose-5-phosphate 3-epimerase is an enzyme that catalyzes the chemical reaction L-ribulose 5-phosphate to L-xylulose 5-phosphate. Hence, RPE has one substrate, L-ribulose 5-phosphate, and one product, L-xylulose 5-phosphate. RPE belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is L-ribulose-5-phosphate 3-epimerase. Other names in common use include L-xylulose 5-phosphate 3-epimerase, UlaE, and SgaU.

Synonym: Ribulose-Phosphate 3-Epimerase, Ribulose-5-Phosphate-3-Epimerase, RPE, HUSSY-17,RPE2-1

Molecular Weight: 27 kDa

NCBI Accession: NP_954699